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This book provides a general introduction of plant tissue culture followed by specific applications of biotechnology in regeneration of rice (Oryza sativa), Maize, Eucalyptus, hot pepper, guava (Psidium guajava L.) stone fruit (Pinus pinea) and compares the features of in vitro grown plants to in vivo plants, Transgenic plants production and application, generating marker-free transgenic plants, genetic engineering and metabolic engineering of plants, molecular farming, abiotic stress tolerance, transgenic in floriculture and ornamental plants, celery, Secondary metabolite production with special reference to sennoside, genetic transformation of potato and biosafety concerns, bioinformatics and its application to crop improvement, Intellectual property rights, biotechnological aspects of secondary metabolite production, application of biotechnology in pharmaceutical sciences and production of recombinant proteins, cyclotides, Hypericum perforatum and Gentiana punctata provide a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. Besides covering basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, gene technology and secondary metabolite production. The book will prove useful to both students and researchers of biotechnology, agriculture, horticulture, forestry as well as for the industry.
1. Tissue Culture: Historical Perspectives and Applications Section I: In Vitro Plant Regeneration 2. High Frequency Plant Regeneration via Multiple Shoot Induction from Shoot Apex in Indica Rice (Oryza sativa L.) Varieties 3. Somatic and Gametic Embryogenesis in Maize: Cell Biology and Applications 4. Somatic Embryogenesis in Eucalyptus—An Update to 2009 5. Increased Embryo Production by Manipulation of Pretreatment Materials and Media in Isolated Microspore Culture of Hot Pepper (Capsicum annuum L.) 6. Biotechnological Advancement in Getting In Vitro Regeneration and Mass Multiplication in Guava (Psidium guajava L.): A Success Story in Fruit Trees 7. In Vitro Propagation of Stone Pine (Pinus pinea L.) Molecular and Physiological Bases 8. Morphological, Anatomical and Physiological Characteristics of in vitro Grown Plants in Comparison to ex vitro Grown Plants Section II: Transgenic Plants and their Applications 9. Production of Transgenic Plants and Their Application in Biotechnology 10. Practical Application of Genetic Transformation 11. Generating Marker-free Transgenic Plants 12. Role of recARad51 Gene Family in Homologous Recombination, Repair and Genetic Engineering of Transgenic Plants 13. Metabolic Engineering in Plants 14. Molecular Farming for Production of Biologically Important Molecules in Plants 15. Improving Crop Tolerance to Abiotic Stresses by Plant Biotechnology 16. Transgenic Floricultural and Ornamental Plants 17. Flux in Morphology and GUS Expression in Somatic Embryogenic-Derived Populations of Transgenic Celery 18. The Potato (Solanum tuberosum L.): An Overview of Genetic Transformation and Related Biosafety Issues 19. Clean DNA Transformation of Rice Using Minimal Gene-Expression-Cassettes to Minimize Biosafety Concerns 20. Bioinformatics Application to Crop Improvement 21. Intellectual Property Rights, Biopiracy & Patent Revocation Section III: Secondary Metabolites and medicinal plants 22. Biotechnological Aspects of Secondary Metabolite Production 23. Applications of Plant Biotechnology in Pharmaceutical Sciences: A Critical Review 24. Production of Recombinant Proteins in Plants for Pharmaceutical Use 25. Intraspecific Variation of Plant Secondary Metabolites 26. Optimisation Steps Towards Bioprocess Development for the Production of Cyclotides 27. Genetic (In)stability in Hypericum perforatum L.: Physiological and Induced Sources of Genetic Variation 28. Biotechnology of Endangered Medicinal Plants: Spotted Gentian, Gentiana punctata L. 29. Direct Shoot Regeneration of Cassia senna for Sustainable Production of Anthraquinone Glycoside-Sennoside Index Author Index
Ashwani Kumar :- Ashwani Kumar, Professor, Department of Botany, University of Rajasthan, Jaipur, has earned gold medal in M.Sc. and obtained Ph.D. from Rajasthan University. He also has the distinction of being an Alexander Von Humboldt Fellow (Germany). He was the Honorary Director at the School of Life Sciences, University of Rajasthan. The author's repertoire of published works spreads across 150 research articles in various national and international journals. With an experience of over three decades in his field of research, namely, tissue culture and biochemistry, the author has been at the helm of various major projects carried out by USDA-ICAR, U.G.C., M.N.E.S., C.S.I.R and DBT. He has long association with Professor Dr. Karl-Hermann Neumann and subsequently with Professor Dr. Sven Schubert at Institute Für Pflanzenernährung, Justus Liebig Universität, Giessen, Germany, as visiting Humboldt Fellow and visiting Professor at Toyama Medical and Pharmaceutical University, Toyama, Japan. The author is currently engaged in research on salinity tolerance at molecular level in cereals and crops. Recently he has been awarded the prestigious V. Puri Medal 2008.Sudhir K. Sopory :- Sudhir K. Sopory, Head of Plant Molecular Biology Division at the International Centre for Genetic Engineering and Biotechnology, New Delhi, is a well-known Plant Biologist who is recognized for his contributions in research and teaching in the field of Molecular Plant Physiology and Plant Biotechnology.He obtained Ph.D. from the University of Delhi. He is a visiting scientist at Max-Planck-Institute, Kolen, and University of Munich, in Germany, and at the University of Texas, Austin and United States Department of Agriculture, Maryland, USA.
Discount : 20.00 %
Discount : 20.00 % Price : 35.00 28.00
Discount : 20.00 %
Price : 35.00 28.00