This book describes the principles of the bioanalytical techniques that are used in clinical diagnosis, pharmaceutical analysis, bioprocess monitoring, environmental studies and life sciences research. The primary focus of this book is on instrumental methods which are evolving rapidly due to the availability of new technologies. The basic concepts of each technique are explained in as concise and simple a manner as possible, without sacrificing generality or rigour.
Part-I and Part-II are primarily for undergraduate students. Part-I describes analytical methods that are based on the separation of particles (chromatography, electrophoresis, mass spectrometry, etc). Part-II describes methods based on spectroscopy, scattering and diffraction. Part-III introduces some basic biochemical and molecular biological methods such as binding assays, PCR, protein sequencing and nucleic acid sequencing and is intended as background material for students who are interested in the topics described in Part-IV. Part-IV describes the basic concepts of novel micro- and nano-bioanalytical techniques which are based on biosensors, microarrays, microfluidics, surface plasmon resonance and nanomaterials. Part-IV is primarily for postgraduate students. However, adequate basics are provided to ensure that advanced undergraduates can also benefit from this section.
Sekhar Talluri :- is a Professor in the Department of Biotechnology, GITAM University. He has more than tenyears of experience in teaching bioanalytical techniques and related subjects to undergraduate and postgraduate students. He obtained his M.Sc. degree from the Indian Institute of Technology, Delhi in 1984 and Ph.D. from Cornell University in 1993 for the thesis entitled "NMR spectroscopic studies of protein unfolding." Subsequently, he served as a research fellow in the Department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School for several years. He has received more than 250 citations for his publications.