Mammalian Cell Technology and Its Applications has been thoroughly revised keeping in view the developments in the recent past in the field of 'Regenerative Medicine'. The book is now divided into five sections consisting of sixteen chapters, which now appears to be most convenient for the diverse backgrounds of students starting from basic biology to the bioengineering discipline. Since, animal cell technology commands proper understanding of cell biology, DNA technology, immunology and bioengineering, the goal of this book is to amalgamate knowledge from these fields and pass these on to the readers who intend to start their professional career in academics or in industrial research. An animal cell is a unique factory, where thousands of genes are encoded and transcribed. Products are translated, and finally processed to biologically active molecules. It is therefore, important to understand the inside of a cell, how cellular functions are coordinated, what are the limitations of a cell, and reasons for proliferation and cellular death. In this revised version, the first two chapters are dedicated to basic cell biology aspects, which will orient the students from other backgrounds. The second section covers basic cell culture technology, which consists of preservation and control of cell lines, cell culture media, good manufacturing practices, cell culture equipment, culture environment and methods, etc. In the third section, recombinant therapeutic proteins, large-scale cell culture, and scale-up processes are discussed. The fourth section has been thoroughly revised providing detailed aspects of cell-based therapeutic applications for human (animal) diseases. The first part of this section covers basic aspects of different 'stem cells' and how they are derived. The next two chapters are dedicated to 'regenerative medicine' where tissue engineering and cell therapy approaches have been elucidated. The last section of the book deals with the analytical techniques used in cell culture practices and protocols for isolation of cells, etc.
Section I: Introduction to Cell Biology1. Organization of Animal Cells and Special Properties2. Proliferation and Death of Animal Cells: Molecular MechanismSection II: Basic Cell Culture Technology3. Sources of Animal Cells, Preservation and Control of Cell4. Design of Cell Culture Media5. Good Manufacturing Practices, Aseptic Techniques, and Cell Culture Equipment6. Environmental Factors and Cell Culture Methods7. Separation of Viable CellsSection III: Therapeutic Proteins and Bioengineering Aspects8. Stable Gene Expression in Mammalian Cells and Monoclonal Antibody9. Large-scale Animal Cell Culture for the Production of Therapeutic Proteins10. Scale-Up Studies in Animal Cell Culture BioreactorsSection IV: Advanced Cell Culture Technology11. Embryonic and Adult Stem Cells12. Regenerative Medicine: Tissue Engineering13. Regenerative Medicine: Cellular Therapy14. Present Trend and Future PerspectiveSection V: Analytical Techniques and Cell Isolation15. Quantitative Analysis16. Establishing Primary Cultures and Cryopreservation of Cells/TissuesGlossaryIndex
Asok Mukhopadhyay :- Asok Mukhopadhyay is a senior scientist at the National Institute of Immunology, New Delhi. His areas of working are stem cell research, tissue engineering, and therapeutic applications of stem cells. He did Master's in Technology and Doctorate in Philosophy in the field of Biochemical Engineering and Biotechnology from Indian Institute of Technology, Delhi, India. He did Postdoctoral Research from MD Anderson Cancer Center, Houston, Texas, USA.