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The Hon'ble Prime Minister Shri Narendra Modi's vision of 100 smart cities needs to be transformed through Indo-Global interventions. This book is an encyclopaedia of studies of various eras of civilization, indicating the innovations in materials, technology and the process of transformation of human settlement. The studies of ethnic, heritage, and sustainable Indian cities - Chandigarh, Jaipur, and global walkable and cyclable cities - London, Paris, and Barcelona, along with the modern smart cities of GIFT, Dholera, and Masdar have been critically analyzed. The author concludes through the detailed study of existing mega city of Delhi to arrive at various parameters helping in redefining Indian sustainable and smart cities of tomorrow.
“This is a very good student friendly manual and I strongly recommend for its publication. I praise the efforts of the authors for bringing out such a manual for which nobody thought earlier.” --Dr Jagat Kumar Roy, Cytogenetics Lab, Banaras Hindu University, India. “This manual combines the study of the most crucial theoretical aspects in Drosophila genetics, discussing a wide spectrum of methods used in the field. Overall, the book is written in a very clear way. It has a consistent and clear structure and the figures and pictures presented are of good quality. I strongly recommend and support the publication of this manual.” --Héctor Herranz, PhD, Associate Professor, Department of Cellular and Molecular Medicine, University of Copenhagen, Denmark Genetics is taught in all life science related disciplines at the undergraduate level. This laboratory manual meant for beginners is an outcome of the authors’ attempt made in the last three years (2012 -15) of designing experiments to teach the principles of transmission genetics using Drosophila as a model system. The purpose of Drosophila resource centre was to demonstrate the ease of learning genetics through experimentation. Wherever required the existing approaches have also complemented the new effort. The experiments are designed to teach the basic principles of genetics in a one-semester course. More than 90% of the exercises were conducted using a handful of Drosophila mutants. Most of the exercises are organized in a similar fashion covering 6-8 pages. Each exercise provides background information with supporting examples from literature; protocol; observations and calculations (if any) and finally results and discussion.
1. Maintenance and culturing of Drosophila 2. Sexing and collection of virgin females 3. To study the life cycle of Drosophila melanogaster 4. To study mutant phenotypes of Drosophila melanogaster 5. To demonstrate Mendel’s law of segregation and dominance 6. To demonstrate Mendel’s law of independent assortment 7. To study the inheritance of white eye colour in Drosophila 8. To map sepia and curl mutants by recombination mapping 9. To demonstrate absence of crossing over in Drosophila males 10. To establish dominance relationship between multiple alleles of a gene 11. To demonstrate that the white-eyed flies have a deposition defect 12. To perform cis-trans test of allelism with the given eye colour mutants 13. To demonstrate dosage compensation in Drosophila melanogaster 14. To create a Bar and apricot eyed double mutant 15. To detect recessive lethal alleles in genetic crosses 16. To study the penetrance and expressivity of serrate and vestigial wings in Drosophila melanogas 17. To study the characteristics of X-linked dominant traits 18. To separate eye pigments (pterins) by thin layer chromatography 19. To prepare stained squashes of salivary glands showing well spread polytene chromosomes20. To demonstrate recombination within a gene 21. To determine the inheritance pattern of white eye colour and vestigial wings from the F2 data 22. To dissect the malphigian tubules, brain and imaginal discs from the third instar Drosophila larva 23. To analyse using bioinformatic tools Drosophila as a model system (Toll 9 gene) for human diseases (asthma) 24. To test for PTC tasting in a random population and to calculate allele and genotype frequency using Hardy Weinberg law 25. To determine the ABO blood groups in a random sample and calculate the allele frequency using Hardy Weinberg law
Sunita Joshi :- Dr. Sunita Joshi is presently an associate professor at Department of Biochemistry, Daulat Ram College, University of Delhi with more than 25 years of teaching experience. She has trained around 100 students from Botany, Biochemistry and Life Sciences in Drosophila Genetics in last three years. She is a resource person at ILLL, DU; DBT; CBSE. She is a co-author of Textbook of Biotechnology, Class XI (CBSE). She is also an educationist member in KVS for the past 5 years and reviewer of courses in Biochemistry offered by IGNOU. Dr. Sunita Joshi is the recipient of M.N Gupta memorial award, 2003.Neeru Dhamija :- Dr. Neeru Dhamija is presently an assistant professor at Department of Biochemistry, Daulat Ram College, University of Delhi. She has M.Sc. in Biochemistry and a doctorate in Biotechnology with specialization in HIV Biology. She studied the role of HIV-1 Tat protein in regulation of host cellular genes, under the guidance of Dr. Debashis Mitra at National Centre for Cell Science. She has trained around 70 students from Botany, Biochemistry and Life Sciences in Drosophila Genetics in last two years. She has also worked as assistant professor at Cluster Innovation Centre, DU. She is also an eContent writer in UGC-ePG Paathshaala (Chemistry).
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