Basic Techniques in Biochemistry and Molecular Biology, 1/e
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Basic Techniques in Biochemistry and Molecular Biology, 1/e

Author : R.K. Sharma & S.P.S. Sangha
© Year : 2008
ISBN : 9788190746267
Pages : 280
Binding : Paperback
Weight : 0.29   kg
Price :   15.00

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  • About the Book
  • Salient Features
  • Table of Contents
  • About the Author

Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.

Salient Features:

  • Spread in 15 chapters, it covers more than 135 experiments ranging from preparation of culture media to PCR, Southern and Western blotting, and a general introductory chapter.
  • Every chapter is preceded with a brief theoretical background, and each experiment with principle/working and requirements.
  • Appendices include standard values, preparation of buffer solutions, vitamin levels, etc.

  • General Introducton
  • pH and Buffers
  • Spectrophotometry
  • Centrifugation and Cell Fractionation
  • Chromatography
  • Carbohydrates
  • Lipids
  • Amino Acids and Proteins
  • Nucleic Acids
  • Electrophoresis
  • Immunology
  • Enzymes
  • Metabolites
  • Vitamins, Hormones and Pigments
  • Molecular Biology
  • Appendices Readership: Students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels.

R.K. Sharma :- Rakesh K. Sharma is Coordinator, Green Chemistry Network Centre and In-charge, International Chapter of the American Chemical Society’s Green Chemistry Institute (GCI), University of Delhi.


S.P.S. Sangha :- S.P.S Sangha, Professor & Head, Department of Biochemistry, Guru Angad Dev University of Veterinary and Animal Sciences, Ludhiana, Punjab.

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